Instant Sticky-end Ligase Master Mix

#M0370

cloned at NEB recombinant No
Catalog # Size Concentration Price (£)
M0370L 250 REACTIONS 2 X £400.00
M0370S 50 REACTIONS 2 X £100.00

Instant Sticky-end Ligase Master Mix will ligate these substrates:

dsDNA




Nicked DNA/RNA





 

Specifically formulated to rapidly ligate cohesive-end (2-4 bp) substrates and improve transformation, Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA Ligase and a proprietary ligation enhancer in an optimized reaction buffer.

  • No incubation time necessary to achieve ligation

  • No thawing of the master mix is required as liquid state is maintained when stored at -20°C

  • Simplified reaction setup with optimized ratio of enzyme and buffer components

  • Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution

  • Other T4 DNA Ligase products include Quick Ligation Kit, Salt-T4, Hi-T4, and Blunt/TA Ligase Master Mix

  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart


Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA ligase and a proprietary ligation enhancer in an optimized reaction buffer. Specifically formulated to rapidly ligate cohesive-end (2-4 bp) substrates and improve transformation, the mix simplifies reaction set-up and provides an optimized ratio of enzyme and buffer components. No thawing of the master mix is required as it maintains a liquid state during storage at -20°C* and no incubation time is necessary to achieve ligation efficiencies sufficient for successful sub-cloning of sticky-end substrates. Just add the master mix to DNA with compatible ends, mix and transform; thereby reducing the valuable time needed for routine ligations. Ligations for subcloning can be carried out in small volumes with low concentrations, allowing users to conserve precious DNA samples, and be used directly to transform many strains of chemically competent E. coli without dilution.

* Freezers vary in their actual internal temperature. Our testing demonstrates that the master mix is liquid at -20°C. Freeze-thaw testing at -70°C has confirmed that the performance after 20 freeze/thaw cycles is close to the original mix.

Product Source

Purified from an E. coli strain containing a recombinant gene encoding T4 DNA Ligase.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • M0370S

     

     

    -20

     

     

  • M0370L

     

     

    -20

     

     

Advantages and Features

Properties & Usage

Related Products

Product Notes

Protocols

Datacards

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



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