T4 DNA Ligase


recombinant NEBU 16 65 Heat
Catalog # Size Concentration Price (£)
M0202L 100000 UNITS 400,000 units/ml £237.00
M0202M 100000 UNITS 2,000,000 units/ml £237.00
M0202S 20000 UNITS 400,000 units/ml £60.00
M0202T 20000 UNITS 2,000,000 units/ml £60.00

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T4 DNA Ligase will ligate these substrates:


Nicked DNA/RNA


For details on NEB's quality controls for DNA ligases, visit our Ligase Quality page.

T4 DNA Ligase Competitor Study - Nuclease Contamination
T4 DNA Ligase Competitor Study- Nuclease ContaminationT4 DNA Ligase from multiple suppliers was tested in reactions containing a fluorescent labeled single stranded, double stranded blunt, 3'overhang or 5' overhang containing oligonucleotides. The percent degradation by contaminating nucleases is determined by capillary electrophoresis and peak analysis. The resolution is at the single nucleotide level.


  • Isolated from a recombinant source

  • Supplied with 10X Reaction Buffer

Product Source

An E. coli strain that carries the cloned T4 DNA Ligase gene.

Reagents Supplied

The following reagents are supplied with this product:


Component Name

Component #

Stored at (°C)



  • M0202S






  • M0202T






  • M0202L






  • M0202M






Advantages and Features

Properties & Usage

Related Products

Product Notes


Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.


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