NEBNext® Poly(A) mRNA Magnetic Isolation Module

#E7490

Catalog # Size Price (£)
E7490L 96 REACTIONS £250.00
E7490S 24 REACTIONS £70.00

Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80-90% of total RNA, and efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples. Oligo dT-based mRNA isolation is ideal for enrichment of mRNA and separation from rRNA when samples are eukaryotic and have intact poly(A) tails.

Description

The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A) RNA from previously isolated total RNA. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A) RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A) transcripts in the eluent. Intact poly(A) RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour.
 
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.


Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)

Concentration

NEBNext RNA Binding Buffer (2X)

4

2 X

NEBNext Wash Buffer

4

Nuclease-free Water

-20

NEBNext Tris Buffer

4

NEBNext Oligo d(T)25 Beads

4

Application Features

Isolation of poly(A) RNA transcript from Total RNA for RNA library preparation and sequencing.


Properties and Usage

Materials Required but not Supplied

  • 96-well 0.2 ml PCR Plates and Microseal® 'B' Adhesive Sealer (BioRad MSB-1001) or 0.2 ml RNase-free tube.

  • Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)

  • Thermal cycler or heat block

  • Bioanalyzer® (Agilent Technologies, Inc.) or similar instrument and consumables.

Storage Temperature

4°C

Protocols
  1. Isolate mRNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)
  2. Protocol for Enrichment of mRNAs, excluding Globin mRNA, from Whole Blood Total RNA

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Quality Control

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.


Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]


Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]


Safety Data Sheet

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science - we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

This product is for research use only, not for therapeutic use, and for use only in nucleic acid purification as part of sample preparation prior to sequencing.



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