The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving you a step. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging
View our videos on protocols, tips, and recycling Monarch.
Monarch DNA Cleanup Column Design
Monarch columns are designed for performance
Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.
Advantages:
-
Elute in as little as 6 μl
-
Prevent buffer retention and salt carry-over with optimized column design
-
Save time with fast, user-friendly protocols
-
No need to monitor pH or add isopropanol
-
Buffers and columns available separately
-
Significantly less plastic used when compared with other kits
-
Responsibly-sourced and recyclable packaging
Specifications
|
DNA Sample Type:
|
double-stranded DNA from agarose gels
|
|
Binding Capacity:
|
up to 5 μg
|
|
DNA Size Range:
|
~50 bp to 25 kb
|
|
Typical Recovery:
|
DNA (50 bp to 10 kb): 70-90%
DNA (11-23 kb): 50-70%
|
|
Elution Volume:
|
≥ 6 μl
|
|
Purity:
|
A260/280 > 1.8
|
|
Protocol Time:
|
10 minutes of spin and incubation time
|
|
Compatible Downstream
Applications:
|
ligation, restriction digestion, labeling and other enzymatic
manipulations, library construction and DNA sequencing.
|
Monarch DNA Gel Extraction Kit Protocol
DNA purified from the Monarch DNA Gel Extraction Kit is recovered with similar efficiency and purity as the leading supplier, but is more highly concentrated, facilitating its use in downstream applications
One microgram aliquots of a 3 kb fragment were resolved on a 1% w/v agarose gel, excised, and processed with different kits using manufacturer-specified minimum elution volumes. Values reported are the concentration and purity data determined by Nanodrop™ readings, as well as recovery calculations based on the eluted DNA concentration and recovered volume. Monarch DNA Gel Extraction Kit reproducibly recovers DNA over a broad range of molecular weights.
A mixture of 7 DNA fragments ranging from 10 kb down to 0.5 kb was prepared and one-half of the mixture was resolved on a 1% gel. Each fragment was manually excised from the agarose gel and processed using the Monarch DNA Gel Extraction Kit. The entire elution of each fragment was resolved on a new gel with the remainder of the original mixture for comparison. DNA purified from agarose gels using the Monarch DNA Gel Extraction Kit can be reproducibly isolated and ligated.
Two micrograms of a 3 kb fragment with compatible ends was resolved on a 1% agarose gel, excised, and purified using the Monarch DNA Gel Extraction Kit. Samples were eluted in 20 μl and a fraction (1/4 th of total) was ligated using the Blunt/TA Ligase Master Mix (NEB #M0367). Representative samples from 5 replicates were resolved on a second 1% agarose gel. M is the 1 kb DNA Ladder (NEB #N3232).
Kit Components
The following reagents are supplied with this product:
|
NEB #
|
Component Name
|
Component #
|
Stored at (°C)
|
Amount
|
Concentration
|
Advantages and Features
Features
-
Elute in as little as 6 μl
-
Prevent buffer retention and salt carry-over with optimized column design
-
Save time with fast, user-friendly protocols
-
No need to monitor pH or add isopropanol
-
Buffers and columns available separately
-
Significantly less plastic used when compared with other kits
-
Responsibly-sourced and recyclable packaging
Properties & Usage
Related Products
Companion Products
Materials Sold Separately
Product Notes
-
The kit should be stored at room temperature. Always keep buffer bottles tightly closed and keep columns sealed in the enclosed zip-lock bag. For information regarding the composition of buffers, please consult the Safety Data Sheets. Proper laboratory safety practices should be employed, including the use of lab coats, gloves and eye protection.
Protocols
Manuals
The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.
Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.
Legal and Disclaimers
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please email us at busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science - we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.