Monarch® DNA Gel Extraction Kit

#T1020

Catalog # Size Price (£)
T1020L 250 PREPS £369.00
T1020S 50 PREPS £83.00

Quickly and easily purify DNA from agarose gels with high yields.

  • Elute in as little as 6 μl

  • Prevent buffer retention and salt carry-over with optimized column design

  • Save time with fast, user-friendly protocols

  • No need to monitor pH or add isopropanol

  • Buffers and columns available separately

  • Significantly less plastic used when compared with other kits 

  • Responsibly-sourced and recyclable packaging

 

Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.

The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving you a step. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging


View our videos on protocols, tips, and recycling Monarch.

Monarch DNA Cleanup Column Design
Monarch DNA Cleanup Column Design Monarch columns are designed for performance
Monarch columns are designed for performance. Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.

Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.
Advantages:

  • Elute in as little as 6 μl

  • Prevent buffer retention and salt carry-over with optimized column design

  • Save time with fast, user-friendly protocols

  • No need to monitor pH or add isopropanol

  • Buffers and columns available separately

  • Significantly less plastic used when compared with other kits

  • Responsibly-sourced and recyclable packaging


Specifications

DNA Sample Type:

double-stranded DNA from agarose gels

Binding Capacity:

up to 5 μg

DNA Size Range:

~50 bp to 25 kb

Typical Recovery:

DNA (50 bp to 10 kb): 70-90%
DNA (11-23 kb): 50-70%

Elution Volume:

≥ 6 μl

Purity:

A260/280 > 1.8

Protocol Time:

10 minutes of spin and incubation time

Compatible Downstream
Applications:

ligation, restriction digestion, labeling and other enzymatic
manipulations, library construction and DNA sequencing.


Monarch DNA Gel Extraction Kit Protocol 
Monarch DNA Gel Extraction Kit Protocol DNA purified from the Monarch DNA Gel Extraction Kit is recovered with similar efficiency and purity as the leading supplier, but is more highly concentrated, facilitating its use in downstream applications
 DNA purified from the Monarch DNA Gel Extraction Kit is recovered with similar efficiency and purity as the leading supplier, but is more highly concentrated, facilitating its use in downstream applications. One microgram aliquots of a 3 kb fragment were resolved on a 1% w/v agarose gel, excised, and processed with different kits using manufacturer-specified minimum elution volumes. Values reported are the concentration and purity data determined by Nanodrop™ readings, as well as recovery calculations based on the eluted DNA concentration and recovered volume.

One microgram aliquots of a 3 kb fragment were resolved on a 1% w/v agarose gel, excised, and processed with different kits using manufacturer-specified minimum elution volumes. Values reported are the concentration and purity data determined by Nanodrop™ readings, as well as recovery calculations based on the eluted DNA concentration and recovered volume. Monarch DNA Gel Extraction Kit reproducibly recovers DNA over a broad range of molecular weights. 
Monarch DNA Gel Extraction Kit reproducibly recovers DNA over a broad range of molecular weights. A mixture of 7 DNA fragments ranging from 10 kb down to 0.5 kb was prepared and one-half of the mixture was resolved on a 1% gel. Each fragment was manually excised from the agarose gel and processed using the Monarch DNA Gel Extraction Kit. The entire elution of each fragment was resolved on a new gel with the remainder of the original mixture for comparison. A mixture of 7 DNA fragments ranging from 10 kb down to 0.5 kb was prepared and one-half of the mixture was resolved on a 1% gel. Each fragment was manually excised from the agarose gel and processed using the Monarch DNA Gel Extraction Kit. The entire elution of each fragment was resolved on a new gel with the remainder of the original mixture for comparison. DNA purified from agarose gels using the Monarch DNA Gel Extraction Kit can be reproducibly isolated and ligated.
DNA purified from agarose gels using the Monarch DNA Gel Extraction Kit can be reproducibly isolated and ligated. Two micrograms of a 3 kb fragment with compatible ends was resolved on a 1% agarose gel, excised, and purified using the Monarch DNA Gel Extraction Kit. Samples were eluted in 20 μl and a fraction (1/4 th of total) was ligated using the Blunt/TA Ligase Master Mix (NEB #M0367 (https://international.neb.com/products/m0367-blunt-ta-ligase-master-mix) ). Representative samples from 5 replicates were resolved on a second 1% agarose gel. M is the 1 kb DNA Ladder (NEB #N3232 (https://international.neb.com/products/n3232-1-kb-dna-ladder) ). Two micrograms of a 3 kb fragment with compatible ends was resolved on a 1% agarose gel, excised, and purified using the Monarch DNA Gel Extraction Kit. Samples were eluted in 20 μl and a fraction (1/4 th of total) was ligated using the Blunt/TA Ligase Master Mix (NEB #M0367). Representative samples from 5 replicates were resolved on a second 1% agarose gel. M is the 1 kb DNA Ladder (NEB #N3232).

 

Kit Components

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • T1020S

     

     

    25

     

     

  • T1020L

     

     

    25

     

     

Advantages and Features

Properties & Usage

Related Products

Product Notes

Protocols

Manuals

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



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