Monarch® PCR & DNA Cleanup Kit (5 μg)

#T1030

Catalog # Size Price (£)
T1030L 250 PREPS £369.00
T1030S 50 PREPS £83.00

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Quickly and easily purify high quality DNA from PCR and other enzymatic reactions.

  • Elute in as little as 6 μl

  • Prevent buffer retention and salt carry-over with optimized column design

  • Save time with fast, user-friendly protocols

  • No need to monitor pH

  • Protocol modification

    allows for ssDNA purification, oligonucleotide purification, and purification of other small DNA fragments

  • Buffers and columns available separately

  • Significantly less plastic used when compared with other kits 

  • Responsibly-sourced and recyclable packaging

Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.

The Monarch PCR & DNA Cleanup Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from PCR and other enzymatic reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging. The protocol can also be modified to enable the purification of smaller DNA fragments, including oligonucleotides and ssDNA.

View our videos on protocols, tips, and recycling Monarch.

 

APPLICATIONS

PCR cleanup

DNA from PCR reactions can be purified after amplification to remove polymerases, primers, detergents, dNTPs, etc.

Enzymatic reaction cleanup

Restriction enzymes and modifying enzymes such as ligases, kinases, nucleases, phosphatases are efficiently removed, allowing for effective desalting and concentration of the DNA sample.

cDNA cleanup

DNA/RNA complexes can be purified post-reverse transcription/amplification to enable removal of the RT and polymerase as well as nucleotides.

Labeling cleanup

Unincorporated radiolabeled or fluorescently labeled nucleotides can be removed from the DNA substrate

Plasmid cleanup

Plasmid preps from unknown sources may contain inhibitors and unwanted contaminants. Purification and concentration can be easily achieved using this kit.

Oligonucleotide cleanup

ssDNA oligonucleotides (≥ 18 nt) and dsDNA fragments (≥ 15 bp) can be purified using the Oligonucleotide Cleanup Protocol.



Monarch DNA Cleanup Column Design
Monarch DNA Cleanup Column Design
Monarch columns are designed for performance
Monarch columns are designed for performance. Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.

Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.
Specifications

DNA Sample Type:

DNA from PCR and other enzymatic reactions (e.g., restriction digests, kinase reactions, ligations).
ssDNA or dsDNA oligonucleotides from enzymatic reactions can also be purified using the Oligonucleotide Cleanup Protocol.

Binding Capacity:

up to 5 μg

DNA Size Range:

~50 bp to 25 kb 
DNA ≥  15 bp to 25 kb (dsDNA) and DNA ≥ 18 nt to 10 kb (ssDNA) can also be purified using the Oligonucleotide Cleanup Protocol.

Typical Recovery:

DNA (50 bp to 10 kb): 70-90%
DNA (11-23 kb): 50-70%
ssDNA ≥  18 nt and dsDNA ≥ 15 bp: 70-85%

Elution Volume:

≥ 6 μl

Purity:

A260/280 > 1.8 and A260/230 > 1.8

Protocol Time:

5 minutes of spin and incubation time

Compatible Downstream
Applications:

ligation, restriction digestion, labeling and other enzymatic
manipulations, library construction and DNA sequencing.


Monarch PCR & DNA Cleanup Kit (5 µg) Protocol 
Monarch PCR & DNA Cleanup Kit (5 µg) Protocol
Monarch PCR & DNA Cleanup Kit (5 μg) performs equivalently to the leading supplier
Monarch PCR & DNA Cleanup Kit (5 μg) performs equivalently to the leading supplier. Preps were performed according to recommended protocols. 1 μg of a 3 kb DNA fragment was incubated with 1 μM primers and OneTaq® Quick-Load® 2X Master Mix (NEB #M0486). DNA was eluted in 20 μl (NEB) and 40 μl (Qiagen) Elution Buffer. Half of the total elution volume was digested with 5 units of DraIII-HF® (NEB #R3510). The digest and the unused portion of the elution were resolved on a 1% w/v agarose gel along with a representative sample of the starting material.

Preps were performed according to recommended protocols. 1 μg of a 3 kb DNA fragment was incubated with 1 μM primers and OneTaq® Quick-Load® 2X Master Mix (NEB #M0486). DNA was eluted in 20 μl (NEB) and 40 μl (Qiagen) Elution Buffer. Half of the total elution volume was digested with 5 units of DraIII-HF® (NEB #R3510). The digest and the unused portion of the elution were resolved on a 1% w/v agarose gel along with a representative sample of the starting material. Monarch PCR & DNA Cleanup Kit (5 μg) removes low molecular weight primers from dsDNA samples
Monarch PCR & DNA Cleanup Kit (5 μg) removes low molecular weight primers from dsDNA samples. Three independent amplicons (267 bp, 520 bp, 1003 bp) were spiked with two oligonucleotides (16-mer, 24-mer) to a final concentration of 1 μM. Half of each mix was purified with the Monarch PCR & DNA Cleanup Kit (5 μg) following the included protocol. Equivalent fractions of the original mixture and the eluted material were resolved on a 20% TBE acrylamide gel at 100V for one hour and stained with SYBR Green II. Three independent amplicons (267 bp, 520 bp, 1003 bp) were spiked with two oligonucleotides (16-mer, 24-mer) to a final concentration of 1 μM. Half of each mix was purified with the Monarch PCR & DNA Cleanup Kit (5 μg) following the included protocol. Equivalent fractions of the original mixture and the eluted material were resolved on a 20% TBE acrylamide gel at 100V for one hour and stained with SYBR Green II.

Kit Components

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • T1030S

     

     

    25

     

     

  • T1030L

     

     

    25

     

     

Advantages and Features

Properties & Usage

Related Products

Product Notes

Quality Control Assays

Certificate of Analysis

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



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