Monarch® RNA Cleanup Kit (10 μg)

#T2030

Catalog # Size Price (£)
T2030L 100 PREPS £278.00
T2030S 10 PREPS £51.00

The Monarch RNA Cleanup Kit (10 µg) enables fast and simple purification and concentration of up to 10 µg of RNA from enzymatic reactions.

  • Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT)

  • Elute in ≥ 6 µl for concentrated RNA

  • 70-100% RNA recovery, even with inputs < 20 ng

  • Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)

  • Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions

  • Simplified workflow with a single wash buffer

  • Unique column design prevents buffer carryover and elution of silica particulates

  • Columns

    and buffers are available separately

  • Purified RNA is ready for use in a wide variety of downstream applications

Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.

The Monarch RNA Cleanup Kit (10 µg) rapidly and reliably purifies up to 10 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nts).

Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.

Monarch RNA Cleanup kits are also available for 50 µg (NEB #T2040) and 500 µg (NEB #T2050) binding capacities. Columns and buffers are also available separately for convenience.

 

Figure 1: Monarch RNA Cleanup Kit workflow

 

Specifications and Applications:

SPECIFICATIONS
RNA Sample Type

Cleanup and concentration of RNA from enzymatic reactions (labeling, capping, in vitro transcription reactions, DNase I treatment)

Binding Capacity

10 μg

RNA Size Range

≥ 25 nt ( ≥ 15 nt with modified protocol)

Typical Recovery

70-100%

Elution Volume

6-20 µl

Purity

A260/280 > 1.8 and A260/230 > 1.8

Protocol Time

5 minutes of spin and incubation time

Compatible Downstream Applications

RT-PCR, Small RNA library prep for NGS, RNA Library Prep for NGS


APPLICATIONS
RNA Cleanup and Concentration (including from the TRIzol aqueous phase)

RNA purified by other methods can be further purified

Enzymatic Reaction Cleanup

Enzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting

In vitro Transcription Cleanup

Enzymes and excess NTPs are removed to yield highly pure synthesized RNA

RNA Gel Extraction

Purification of RNA from agarose gels

RNA Fractionation

Fractionation of RNA into small and large RNA pools

 

Figure 2: The Monarch RNA Cleanup Kit enables efficient recovery of RNA in as little as 6 µl

rRNA (10 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch RNA Cleanup Kit (10 µg, NEB #2030) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A260 as measured using a Trinean DropSense 16. ~80% of RNA can be efficiently recovered in as little as 6 µl. Figure 3: The Monarch RNA Cleanup Kit enables efficient recovery of <20 ng of RNA in as little as 6 µl

A 2-fold dilution series (from 1000 to 15.6 ng) of rRNA (16S and 23S Ribosomal Standard from E.coli, Sigma) was purified using the Monarch RNA Cleanup Kit (10 µg, NEB #1030) and eluted in 6 µl of nuclease-free water. The percent recovery of RNA was calculated from the resulting A260, measured using a Trinean DropSense 16. Even low RNA inputs (<20 ng) are efficiently cleaned up and recovered (>80%) in as little as 6 µl.   Figure 4: The Monarch RNA Cleanup Kit (10 µg) offers efficient recovery of sample volume

 

The Monarch RNA Cleanup Columns (10 µg, NEB #T2037) ensure zero buffer retention and no carryover of contaminants, enabling sample elution in volumes as low as 6 µl. Moreover, ~90% (~5.4 µl) of the minimal elution volume is recovered from the Monarch RNA Cleanup Columns, compared to other competitor kits.

Kit Components

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • T2030S

     

     

    25

     

     

  • T2030L

     

     

    25

     

     

Properties & Usage

FAQs

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



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