Monarch® RNA Cleanup Kit (50 μg)

#T2040

Catalog # Size Price (£)
T2040L 100 PREPS £274.00
T2040S 10 PREPS £49.00

The Monarch RNA Cleanup Kit (50 µg) enables fast and simple purification and concentration of up to 50 µg of RNA from enzymatic reactions.

  • Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT)

  • Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)

  • Elute in ≥ 20 µl for concentrated RNA

  • 70-100% RNA recovery

  • Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions

  • Simplified workflow with a single wash buffer

  • Unique column design prevents buffer carryover and elution of silica particulates

  • Columns

     and buffers are available separately

  • Purified RNA is ready for use in a wide variety of downstream applications, including transfection

Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.

The Monarch RNA Cleanup Kit (50 µg) rapidly and reliably purifies up to 50 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 20 μl. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA library prep for NGS and RNA labeling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nts).

Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.

Monarch RNA Cleanup kits are also available for 10 µg (NEB #T2030) and 500 µg (NEB #T2050) binding capacities. Columns and buffers are also available separately for convenience.

 

Figure 1: Monarch RNA Cleanup Kit workflow

 

Specifications and Applications::

SPECIFICATIONS
RNA Sample Type

Cleanup of previously purified RNA (TRIzol, phenol/ chloroform) and RNA from enzymatic reactions (in vitro transcription reactions, DNase I treatment)

Binding Capacity

50 µg

RNA Size Range

≥ 25 nt ( ≥ 15 nt with modified protocol)

Typical Recovery

70-100%

Elution Volume

20-50 µl

Purity

A260/280 > 1.8 and A260/230 > 1.8

Protocol Time

5 minutes of spin and incubation time

Compatible Downstream Applications

RT =-PCR, RNA library prep for NGS, Formation of ribonucleoprotein (RNP) complexes for genome editing studies, microinjection, RNA labeling, transfection


APPLICATIONS
RNA Cleanup and Concentration (including from the TRIzol aqueous phase)

RNA purified by other methods can be further purified

Enzymatic Reaction Cleanup

Enzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting

In vitro Transcription Cleanup

Enzymes and excess NTPs are removed to yield highly pure synthesized RNA

RNA Gel Extraction

Purification of RNA from agarose gels

RNA Fractionation

Fractionation of RNA into small and large RNA pools

 

Figure 2: The Monarch RNA Cleanup Kit enables efficient recovery of RNA in as little as 20 µl

rRNA (50 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch RNA Cleanup Kit (50 µg, NEB #2040) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A260, as measured using a Trinean DropSense™ 16. Approximately 80% of RNA can be efficiently recovered in as little as 20 µl. Figure 3: The Monarch RNA Cleanup Kit (50 µg) produces sgRNA yields consistent with other competitor RNA cleanup kits and with lower residual NTP contamination

Six different sgRNA synthesis reactions from the EnGen® sgRNA Synthesis Kit, S. pyogenes (NEB #E3322) were cleaned up using either the Monarch RNA Cleanup Kit (50 µg, NEB #T2040) or a competitor kit (according to manufacturer's recommendations) and eluted with 50 µl of nuclease-free water. sgRNA yield was calculated from the resulting A260, as measured using a Trinean Dropsense™ 16. The Monarch Cleanup Kit produced sgRNA yields consistent with other commercially available RNA cleanup kits.

Following cleanup, residual nucleotides (NTPs) were measured by LC-MS and are reported as (percent area NTPs (rATP rCTP rGTP rUTP)/percent area sgRNA). The Monarch RNA Cleanup Kit consistently outperforms other commercially available RNA cleanup kits in the removal of residual NTPs from sgRNA synthesis reactions.  

Kit Components

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • T2040S

     

     

    25

     

     

  • T2040L

     

     

    25

     

     

Properties & Usage

FAQs

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



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