Monarch^® RNA Cleanup Kit (50 μg)
#T2040

Catalog #	Size	Price (£)
T2040L	100 PREPS	£274.00
T2040S	10 PREPS	£49.00
The Monarch RNA Cleanup Kit (50 µg) enables fast and simple purification and concentration of up to 50 µg of RNA from enzymatic reactions. Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT) Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt) Elute in ≥ 20 µl for concentrated RNA 70-100% RNA recovery Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions Simplified workflow with a single wash buffer Unique column design prevents buffer carryover and elution of silica particulates Columns and buffers are available separately Purified RNA is ready for use in a wide variety of downstream applications, including transfection Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.

Catalog #

Size

Price (£)

T2040L

100 PREPS

£274.00

T2040S

10 PREPS

£49.00

The Monarch RNA Cleanup Kit (50 µg) enables fast and simple purification and concentration of up to 50 µg of RNA from enzymatic reactions.

Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT)
Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
Elute in ≥ 20 µl for concentrated RNA
70-100% RNA recovery
Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions
Simplified workflow with a single wash buffer
Unique column design prevents buffer carryover and elution of silica particulates
Columns
and buffers are available separately
Purified RNA is ready for use in a wide variety of downstream applications, including transfection

Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.

The Monarch RNA Cleanup Kit (50 µg) rapidly and reliably purifies up to 50 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 20 μl. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA library prep for NGS and RNA labeling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nts).

Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.

Monarch RNA Cleanup kits are also available for 10 µg (NEB #T2030) and 500 µg (NEB #T2050) binding capacities. Columns and buffers are also available separately for convenience.

Figure 1: Monarch RNA Cleanup Kit workflow

Specifications and Applications::

SPECIFICATIONS
RNA Sample Type	Cleanup of previously purified RNA (TRIzol, phenol/ chloroform) and RNA from enzymatic reactions (in vitro transcription reactions, DNase I treatment)
Binding Capacity	50 µg
RNA Size Range	≥ 25 nt ( ≥ 15 nt with modified protocol)
Typical Recovery	70-100%
Elution Volume	20-50 µl
Purity	A_260/280 > 1.8 and A_260/230 > 1.8
Protocol Time	5 minutes of spin and incubation time
Compatible Downstream Applications	RT =-PCR, RNA library prep for NGS, Formation of ribonucleoprotein (RNP) complexes for genome editing studies, microinjection, RNA labeling, transfection

APPLICATIONS
RNA Cleanup and Concentration (including from the TRIzol aqueous phase)	RNA purified by other methods can be further purified
Enzymatic Reaction Cleanup	Enzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting
In vitro Transcription Cleanup	Enzymes and excess NTPs are removed to yield highly pure synthesized RNA
RNA Gel Extraction	Purification of RNA from agarose gels
RNA Fractionation	Fractionation of RNA into small and large RNA pools

Figure 2: The Monarch RNA Cleanup Kit enables efficient recovery of RNA in as little as 20 µl

rRNA (50 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch RNA Cleanup Kit (50 µg, NEB #2040) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A260, as measured using a Trinean DropSense™ 16. Approximately 80% of RNA can be efficiently recovered in as little as 20 µl. Figure 3: The Monarch RNA Cleanup Kit (50 µg) produces sgRNA yields consistent with other competitor RNA cleanup kits and with lower residual NTP contamination

Six different sgRNA synthesis reactions from the EnGen® sgRNA Synthesis Kit, S. pyogenes (NEB #E3322) were cleaned up using either the Monarch RNA Cleanup Kit (50 µg, NEB #T2040) or a competitor kit (according to manufacturer's recommendations) and eluted with 50 µl of nuclease-free water. sgRNA yield was calculated from the resulting A₂₆₀, as measured using a Trinean Dropsense™ 16. The Monarch Cleanup Kit produced sgRNA yields consistent with other commercially available RNA cleanup kits.

Following cleanup, residual nucleotides (NTPs) were measured by LC-MS and are reported as (percent area NTPs (rATP rCTP rGTP rUTP)/percent area sgRNA). The Monarch RNA Cleanup Kit consistently outperforms other commercially available RNA cleanup kits in the removal of residual NTPs from sgRNA synthesis reactions.

Kit Components

The following reagents are supplied with this product:

Show all

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

T2040S

Monarch^® RNA Cleanup Columns (50 μg)	T2047-21	25	1 x 10 columns	Not Applicable
Monarch^® Collection Tubes II	T2018-21	25	1 x 10 tubes	Not Applicable
Monarch^® RNA Cleanup Binding Buffer	T2041-21	25	1 x 4 ml	Not Applicable
Monarch^® RNA Cleanup Wash Buffer	T2042-21	25	1 x 2.5 ml	Not Applicable
Nuclease-free Water	B1500-21	25	1 x 2.5 ml	Not Applicable

T2040L

Monarch^® RNA Cleanup Columns (50 μg)	T2047-1	25	2 x 50 columns	Not Applicable
Monarch^® Collection Tubes II	T2018-1	25	2 x 50 tubes	Not Applicable
Monarch^® RNA Cleanup Binding Buffer	T2041-2	25	1 x 40 ml	Not Applicable
Monarch^® RNA Cleanup Wash Buffer	T2042-2	25	2 x 20 ml	Not Applicable
Nuclease-free Water	B1500-2	25	1 x 25 ml	Not Applicable

Properties & Usage

FAQs

Protocols

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

manualT2030_T2040_T2050

Usage Guidelines

Quality Control Assays

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

T2040S_L_v2

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please email us at busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science - we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.