Monarch® Total RNA Miniprep Kit

#T2010

Catalog # Size Price (£)
T2010S 50 PREPS £228.00

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Quickly and easily purify up to 100 µg of high quality total RNA from multiple sample types - all with one kit!

  • For use with blood, cells and tissues, saliva, and buccal/nasopharyngeal swabs

  • Also works with tough to lyse samples (bacteria, yeast, plant)

  • Effectively purifies total RNA of all sizes, including small RNAs >20 nt

  • Efficient genomic DNA removal (column and DNase I-based)

  • Contains Proteinase K for processing of tissues and blood samples

  • Includes DNA/RNA Protection Reagent for sample preservation

  • Protocols available for automated RNA extraction from saliva on the QIAcube® and KingFisher Flex

  • Kit components available separately

Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.


The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, tough-to-lyse samples, such as bacteria, yeast, and plant, can be processed with additional steps that enhance lysis. Cleanup of enzymatic reactions or purification of RNA from TRIzol® -extracted samples is also possible using this kit. Purified RNA has high quality metrics, including A260/280 and A260/230 ratios >1.8, high RIN scores, and minimal residual gDNA. Captured RNA ranges in size from full-length rRNAs down to intact miRNAs. Additionally, differential binding conditions allow selective capture or exclusion of the sub-200 nucleotide RNA pool that includes miRNA, 5S rRNA, and tRNA. Purified RNA is suitable for downstream applications, such as RT-qPCR, cDNA synthesis, RNA-seq, Northern blot analysis, etc.

Workflow for the Monarch Total RNA Miniprep Kit

 

Specifications:

Binding Capacity:

100 µg RNA

RNA Size:

≥ 20 nt

Purity:

A260/280 usually ≥ 1.8, A260/230 usually ≥ 1.8

Tissue Input:

up to 20 mg for high yield tissues

 

up to 50 mg for low yield tissues

Cultured Mammalian Cells and Blood Cells (PBMC's, WBC's) Input:

up to 1 x 107

Mammalian Whole Blood Input:

up to 3 ml

Bacteria Input:

up to 1 x 109

Yeast Input:

up to 5 x 107

Plant Input:

up to 100 mg

Elution Volume:

30 - 100 μl

Yield:

varies depending on sample type

Compatible downstream applications:

RNA Library prep for NGS, RT-PCR, RT-qPCR, Northern blots

 

Validated Sample Types:

  • HeLa Cells

  • HEK 293 Cells

  • NIH 3T3 Cells

  • Human Blood

  • Rat Blood

  • PBMC's

  • Plasma

  • Serum

  • Nucleated Blood

  • Rat Liver

  • Rat Spleen

  • Rat Kidney

  • Rat Brain

  • Rat Muscle

  • Mouse Muscle

  • Mouse Heart

  • Mouse Kidney

  • Zebrafish Larvae

  • D. melanogaster
  • S. cerevisiae
  • E. coli
  • B. cereus
  • Tomato Leaf

  • Corn Leaf

  • Saliva

  • Buccal cells

 

Additional Data Figures:

Figure 1. Monarch-purified RNA is high-quality and compatible with a wide variety of downstream applications

monarch
Total RNA from a broad array of sample types was purified using the Monarch Total RNA Miniprep Kit (NEB #T2010). Aliquots were run on an Agilent® Bioanalyzer® 2100 using the Nano or Pico 6000 RNA chip (S. cerevisiae RNA was run using a plant Nano assay). RIN values and O.D. ratios confirm the overall integrity and purity of the RNA. To demonstrate compatibility with downstream applications, samples were subsequently used for RT-PCR (A) using Protoscript® II Reverse Transcriptase (NEB #M0368)/LongAmp® Taq DNA Polymerase (NEB #M0323), NGS library prep (B) using NEBNext® Ultra™ II RNA Library Prep Kit (NEB #E7760) and RT-qPCR (C) using Luna qPCR Reagents (NEB #E3005).

 

Figure 2. The Monarch Total RNA Miniprep Kit can generate high quality RNA from as few as 100 HeLa cells

monarch Total RNA was isolated using the Monarch Total RNA Miniprep Kit (NEB #T2010) from varying amounts of HeLa cells over 5 orders of magnitude and eluted in 100 µl of nuclease-free water. Samples were analyzed on a Bioanalyzer Pico chip, with RIN values and total yields shown below the lanes (A). Electropherograms are included as a reference (B).

 

Figure 3. The Monarch Total RNA Miniprep Kit successfully purifies small RNAs below 200 nucleotides, enabling a more faithful representation of the total RNA pool

monarch
RNA preps were performed on HEK293 cells, mouse heart, rat brain, or rat spleen using the Monarch Total RNA Miniprep Kit (NEB #T2010) and the RNeasy Mini Kit from Qiagen. Equivalent amounts were resolved on a Bioanalyzer 2100 using the Small RNA chip. Monarch-purified RNA contains significantly more RNA in the sub-200 nucleotide pool.

 

Kit Components

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • T2010S

     

     

    25

     

     

Properties & Usage

Related Products

Product Notes

FAQs

Protocols

Manuals

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



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