Quick-Load Purple 1 kb Plus DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing one tracking dye. Tracking dye does not leave a shadow under UV light.
Source
Quick-Load Purple 1 kb Plus DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands.
1 kb Plus DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel.
Mass values are for 1 µg/lane.
Suggested Load: 5 to 10 µl/lane.
Reagents Supplied
The following reagents are supplied with this product:
|
NEB #
|
Component Name
|
Component #
|
Stored at (°C)
|
Amount
|
Concentration
|
Properties & Usage
Bases
|
Fragment
|
Mass (ng)
|
bp
|
|
1
|
40
|
10,002
|
|
2
|
40
|
8,001
|
|
3
|
48
|
6,001
|
|
4
|
40
|
5,001
|
|
5
|
32
|
4,001
|
|
6
|
120
|
3,001
|
|
7
|
40
|
2,017
|
|
8
|
57
|
1,517
|
|
9
|
45
|
1,200
|
|
10
|
122
|
1,000
|
|
11
|
34
|
900
|
|
12
|
31
|
800
|
|
13
|
27
|
700
|
|
14
|
23
|
600
|
|
15
|
124
|
500/517
|
|
16
|
49
|
400
|
|
17
|
37
|
300
|
|
18
|
32
|
200
|
|
19
|
61
|
100
|
Effective Size Range
100bp to 10,002bp
Storage Buffer
2.5% Ficoll®-400
10 mM EDTA
3.3 mM Tris-HCl
0.001% Dye 2
0.02% Dye 1
pH 8 @ 25°C
Related Products
Product Notes
-
Preparation of DNA
The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
Usage recommendation
We recommend loading 5-10 ul (0.5-1 ug) of Quick-Load Purple 1 kb Plus DNA Ladder per gel lane. The Quick-Load Purple 1 kb Plus DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
-
Quick-Load Purple 1 kb Plus DNA Ladder is stable for at least 6 months at 25°C.
For long term storage, store at 4°C or -20°C.
If stored at -20°C, mix well after thawing. If not well mixed, the load may float out of the wells.
References
-
Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular cloning: A Laboratory Manual. (2nd ed.), (pp. 10.51-10.67). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
Selection Charts
Selection Charts
Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.
Legal and Disclaimers
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please email us at busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science - we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.