Gel Loading Dye, Purple (6X) is a pre-mixed loading buffer which contains a combination of two dyes, Dye 1 (pink/red) and Dye 2 (blue). The red dye serves as the tracking dye for both agarose and non-denaturing polyacrylamide gel electrophoresis. The two dyes separate upon gel electrophoresis; the red band is the major indicator and migrates similarly to Bromophenol Blue on agarose gels. Specifically chosen, this dye does not leave a shadow under UV light. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to DNA following cleavage. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. The dye also contains Ficoll, which creates brighter and tighter bands when compared to glycerol loading dyes. This product is packaged as 4x1 ml vials.
Attention SYBR® Safe and GelRed™ dye users: Due to an increased concentration of SDS* in B7024S, NEB recommends using Gel Loading Dye, Purple, No SDS (6X) (NEB# B7025S) instead.
*Compared to NEB# B7021 and NEB# B7022.
Comparison of dye fronts
Nucleic acid samples were run on an agarose gel, with either Purple or Blue loading dyes. Both dyes ran at the same speed. UV shadow comparison
The new Gel Loading Dye, Purple (6X) (Lane 1) included in the Quick-Load Purple 1kb DNA Ladder does not cast a UV shadow over the underlying bands, unlike the Gel Loading Dye, Blue (6X) (Lane 2).
Reagents Supplied
The following reagents are supplied with this product:
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Advantages and Features
Features
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No UV shadow allows for publication grade images
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Brighter, sharper bands vs. glycerol-based loading dyes
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Stops enzyme reactions
Properties & Usage
1X Buffer Components
2.5% Ficoll®-400
10 mM EDTA
3.3 mM Tris-HCl
0.08% SDS
0.02% Dye 1
0.001% Dye 2
pH 8@25°C
Related Products
Product Notes
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Use 5 μl of Gel Loading Dye, Purple (6X) per 25 μl reaction, or 10 μl per 50 μl reaction. Mix well before loading gel.
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Attention SYBR® Safe and GelRed™ dye users:
Due to an increased concentration of SDS in NEB #B7024, NEB recommends using Gel Loading Dye, Purple, No SDS (6X), NEB #B7025 instead.
Protocols
Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.
Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
Legal and Disclaimers
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science - we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Trademarks
SYBR® is a registered trademark of Life Technologies Corp.
GelRed™ is a trademark of Biotium.