Catalog # Size Concentration Price (£)
R0539L 1500 UNITS 10,000 units/ml £257.00
R0539S 300 UNITS 10,000 units/ml £64.00

BbsI has a High Fidelity version BbsI-HF® (NEB #R3539) that can be stored at -20°C.

High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

For details on NEB's quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page.


Product Source

An E. coli strain that carries the cloned BbsI gene from Bacillus brevis.

Reagents Supplied

The following reagents are supplied with this product:


Component Name

Component #

Stored at (°C)



  • R0539S






  • R0539L






Properties & Usage

Related Products

Product Notes

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.

Legal and Disclaimers