Catalog # Size Concentration Price (£)
R3539L 1500 UNITS 20,000 units/ml £257.00
R3539M 1000 UNITS 50,000 units/ml £138.00
R3539S 300 UNITS 20,000 units/ml £64.00

High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart Buffer).  All HF-restriction enzymes come with Gel Loading Dye, Purple (6X).  Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:

Product Source

An E. coli strain that carries the cloned and modified BbsI gene from Bacillus brevis.

Reagents Supplied

The following reagents are supplied with this product:


Component Name

Component #

Stored at (°C)



  • R3539S






  • R3539L






  • R3539M






Properties & Usage

Related Products

Product Notes

Quality Control Assays

Certificate of Analysis

Change Notifications

BbsI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10119525. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin)

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.

Legal and Disclaimers