Catalog # Size Concentration Price (£)
R3733L 5000 UNITS 20,000 units/ml £257.00
R3733S 1000 UNITS 20,000 units/ml £64.00

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Attention Golden Gate Assembly users: BsaI-HFv2 (NEB #R3733) has been optimized for Golden Gate Assembly*.  BsaI-HFv2 also works well for any protocol requiring DNA cutting by BsaI. This is the recommended enzyme for any purpose requiring digestion at the recognition sequence ...5′-GGTCTC(N1)/(N5)-3′...
 
*The requirements for digestion of DNA during Golden Gate Assembly are more demanding than what is encountered in traditional DNA cloning. For Golden Gate, the enzyme must function well in a buffer that might not be the same buffer as that normally provided for the restriction enzyme, and maintain activity for an extended time at elevated temperatures in a dynamic cutting/re-ligating reaction with competition for substrate binding between the endonuclease and ligase. Extensive testing has demonstrated superior performance of BsaI-HFv2 compared to both BsaI (NEB #R0535) and BsaI-HF (NEB #R3535) in this challenging Golden Gate assembly context where restriction enzyme efficiency and fidelity are critically important.

High-Fidelity (HF®) restriction enzymes

have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart™ Buffer).  All HF-restriction enzymes come with Gel Loading Dye, Purple (6X).  Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:



  • Engineered for improved performance

  • Used in Golden Gate Assembly; maintains high activity levels in T4 DNA Ligase Buffer often used in this application

  • Type IIS restriction enzymes

    recognize asymmetric DNA sequences and cleave outside of their recognition sequence

  • 100% activity in rCutSmart Buffer

  • Time-Saver™

    qualified for digestion in 5-15 minutes

  • Reduced star activity

  • NEB has developed convenient kits (using BsmBI-v2 and BsaI-HFv2) for performing Golden Gate Assembly.

  • Restriction Enzyme Cut Site: GGTCTC(1/5)


"I really, really love BsaI-HFv2. We are back to getting phenomenal GGA results again with very little background."

-M.C, Principal Investigator, Davidson College, NC


Product Source

An E. coli strain that carries the cloned and modified BsaI gene from Bacillus stearothermophilus 6-55 (Z. Chen)

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • R3733S

     

     

    -20

     

     

  • R3733L

     

     

    -20

     

     

Properties & Usage

Related Products

Product Notes

FAQs

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



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