Catalog # Size Concentration Price (£)
R3162L 10000 UNITS 20,000 units/ml £235.00
R3162M 10000 UNITS 100,000 units/ml £235.00
R3162S 2000 UNITS 20,000 units/ml £58.00

High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart Buffer).  All HF-restriction enzymes come with Gel Loading Dye, Purple (6X) .  Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:

  • Engineered for improved performance

  • 100% activity in rCutSmart Buffer

  • Time-Saver™

    qualified for digestion in 5-15 minutes

  • Reduced star activity

  • Supplied with 1 vial of Gel Loading Dye, Purple (6X)

  • Restriction Enzyme Cut Site: G/GTNACC

High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.

Restriction Enzyme Competitor Study: Nuclease Contamination

EcoRI, NotI, and BamHI from multiple suppliers were tested in reactions containing a fluorescent labeled single stranded, double stranded blunt, 3'overhang or 5' overhang containing oligonucleotides. The percent degradation is determined by capillary electrophoresis and peak analysis. The resolution is at the single nucleotide level.

Product Source

An E. coli strain that carries the cloned and modified BstEII-HF gene from Bacillus stearothermophilus ET (N. Welker)

Reagents Supplied

The following reagents are supplied with this product:


Component Name

Component #

Stored at (°C)



  • R3162S






  • R3162L






  • R3162M






Properties & Usage

Related Products

Product Notes

Quality Control Assays

Certificate of Analysis

Change Notifications

BstEII-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10133984. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.

Legal and Disclaimers