E. coli Poly(A) Polymerase

#M0276

NEBU recombinant 37
Catalog # Size Concentration Price (£)
M0276L 500 UNITS 5,000 units/ml £265.00
M0276S 100 UNITS 5,000 units/ml £66.00

Poly(A) Polymerase catalyzes the template independent addition of AMP from ATP to the 3´ end of RNA

  • Isolated from a recombinant source

  • Applications include 3' labeling of RNA with ATP or Cordycepin and poly(A) tailing of RNA for cloning or affinity purification

  • Enhances translation of RNA transferred into eukaryotic cells

  • Tested for the absence of DNases and RNases

Poly(A) Polymerase catalyzes the template independent addition of AMP from ATP to the 3´ end of RNA.

Figure 1. Analysis of capped and polyadenylated RNA
Figure 1 A. Agilent Bioanalyzer® analysis of capped and polyadenylated RNA. Longer tails are produced by increasing the enzyme concentration in the reaction. Calculated A-tail lengths are indicated over each lane. Lanes: L: size marker,1: No poly-A tail, 2: 5 units, 3 :15 units, 4 : 25 units of E. coli Poly(A) Polymerase. B. Effect of enzymatic A-tailing on the luciferase reporter activity of CLuc mRNA

Product Source

An E. coli strain that carries the cloned Poly(A) Polymerase gene from 

Advantages and Features

Properties & Usage

Product Notes

References

Selection Charts

Selection Charts

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



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