Poly(A) Polymerase catalyzes the template independent addition of AMP from ATP to the 3´ end of RNA.
Figure 1. Analysis of capped and polyadenylated RNA
A. Agilent Bioanalyzer® analysis of capped and polyadenylated RNA. Longer tails are produced by increasing the enzyme concentration in the reaction. Calculated A-tail lengths are indicated over each lane. Lanes: L: size marker,1: No poly-A tail, 2: 5 units, 3 :15 units, 4 : 25 units of E. coli Poly(A) Polymerase. B. Effect of enzymatic A-tailing on the luciferase reporter activity of CLuc mRNA
An E. coli strain that carries the cloned Poly(A) Polymerase gene from
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