E. coli Poly(A) Polymerase

#M0276

recombinant NEBU 37
Catalog # Size Concentration Price (£)
M0276L 500 UNITS 5,000 units/ml £265.00
M0276S 100 UNITS 5,000 units/ml £66.00

Poly(A) Polymerase catalyzes the template independent addition of AMP from ATP to the 3´ end of RNA

  • Isolated from a recombinant source

  • Applications include 3' labeling of RNA with ATP or Cordycepin and poly(A) tailing of RNA for cloning or affinity purification

  • Enhances translation of RNA transferred into eukaryotic cells

  • Tested for the absence of DNases and RNases

Poly(A) Polymerase catalyzes the template independent addition of AMP from ATP to the 3´ end of RNA.

Figure 1. Analysis of capped and polyadenylated RNA
Figure 1 A. Agilent Bioanalyzer® analysis of capped and polyadenylated RNA. Longer tails are produced by increasing the enzyme concentration in the reaction. Calculated A-tail lengths are indicated over each lane. Lanes: L: size marker,1: No poly-A tail, 2: 5 units, 3 :15 units, 4 : 25 units of E. coli Poly(A) Polymerase. B. Effect of enzymatic A-tailing on the luciferase reporter activity of CLuc mRNA

Product Source

An E. coli strain that carries the cloned Poly(A) Polymerase gene from E. coli (1).

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • M0276S

     

     

    -20

     

     

  • M0276L

     

     

    -20

     

     

Advantages and Features

Properties & Usage

Product Notes

References

Protocols

Datacards

Selection Charts

Selection Charts

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



Datacards

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