The ssRNA Ladder is a set of 7 RNA molecules produced by in vitro transcription of a mixture of 7 linear DNA templates. The ladder sizes are: 9000, 7000, 5000, 3000, 2000, 1000 and 500 bases. The 3000 base fragment is at double intensity to serve as a reference band. This ladder is suitable for use as an ssRNA size standard on denaturing or native agarose gels.
Usage Recommendation
This marker was not designed for precise quantification of ssRNA mass.
Denaturing vs. Native Agarose Gels
It is common practice to electrophorese RNA on a fully denaturing agarose gel, such as one containing formaldehyde (1). However, in many cases it is possible to run RNA on a native agarose gel and obtain suitable results. In fact, it has been demonstrated that treatment of RNA samples in a denaturing buffer maintains the RNA molecules in a denatured state, during electrophoresis, for at least 3 hours (2,3). The use of native agarose gels eliminates (see other side) problems associated with toxic chemicals and the difficulties encountered when staining and blotting formaldehyde gels.
Sample Preparation
The ssRNA Ladder is also compatible with formaldehyde-based loading buffers.
4 µg/lane. 1.0% TBE agarose gel.
Properties & Usage
Bases
|
Fragment
|
bp
|
|
1
|
9,000
|
|
2
|
7,000
|
|
3
|
5,000
|
|
4
|
3,000
|
|
5
|
2,000
|
|
6
|
1,000
|
|
7
|
500
|
Effective Size Range
500bp to 9,000bp
Storage Buffer
20 mM sodium citrate
1 mM EDTA
pH 6 @ 25°C
Related Products
Companion Products
Materials Sold Separately
Product Notes
-
Minimize repeated freeze-thawcycles. It is best to aliquot the marker into singleuse portions.
-
To avoid ribonuclease contamination: wear gloves,use RNase-free water for gels and buffers, washequipment with detergent and rinse thoroughlywith RNase-free water.
References
-
Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, (2nd ed.).
-
Liu, Y.-C. and Chou, Y.-C. (1990). Biotechniques. 9,
-
Cook, S. and Marchetti, C. Unpublished observation
Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.
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Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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