ssRNA Ladder

#N0362

Catalog # Size Price (£)
N0362S 25 GEL LANES £67.00
  • Size range: 500 bases - 9,000 bases
  • Compatible with denaturing and native gels

 

The ssRNA Ladder is a set of 7 RNA molecules produced by in vitro transcription of a mixture of 7 linear DNA templates. The ladder sizes are: 9000, 7000, 5000, 3000, 2000, 1000 and 500 bases. The 3000 base fragment is at double intensity to serve as a reference band. This ladder is suitable for use as an ssRNA size standard on denaturing or native agarose gels.

Usage Recommendation
This marker was not designed for precise quantification of ssRNA mass.

Denaturing vs. Native Agarose Gels
It is common practice to electrophorese RNA on a fully denaturing agarose gel, such as one containing formaldehyde (1). However, in many cases it is possible to run RNA on a native agarose gel and obtain suitable results. In fact, it has been demonstrated that treatment of RNA samples in a denaturing buffer maintains the RNA molecules in a denatured state, during electrophoresis, for at least 3 hours (2,3). The use of native agarose gels eliminates (see other side) problems associated with toxic chemicals and the difficulties encountered when staining and blotting formaldehyde gels.

Sample Preparation
The ssRNA Ladder is also compatible with formaldehyde-based loading buffers.

N0362-photo 4 µg/lane. 1.0% TBE agarose gel.

Properties & Usage

Related Products

Product Notes

References

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



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