Partner with NEB to Incorporate LAMP in your Diagnostics Products
Loop Mediated Isothermal Amplification (LAMP) is an isothermal amplification method designed to detect a target nucleic acid without requiring sophisticated equipment. LAMP provides high sensitivity (to fg or <10 copies of target) but with rapid results: reactions can be performed in as little as 5–10 minutes. Detection of RNA targets is accomplished by simple addition of a reverse transcriptase to the LAMP reaction, with RT-LAMP performed as a true one-step, isothermal workflow.
Products with unique benefits and flexibility
- WarmStart® for easy setup – WarmStart enzymes are inactive at room temperature, and activated when the reaction is warmed above approximately 40°C.
- Reactions can be performed with limited resources, using a water bath for incubation and detection of results by eye, or with real-time measurement and high-throughput instruments.
- Reagents can be purchased as assay kits or as stand-alone enzymes, master mixes and detection dye
- Lyophilization-compatible – most master mixes and enzymes are available in custom glycerol-free formulation
- NEB LAMP reagents support multiple detection methods including turbidity detection, real-time fluorescence detection (when used with LAMP fluorescent dye) and end-point visualization such as colorimetric detection via a metal indicator (e.g., hydroxynaphthol blue.)
Multiple Applications
LAMP has been proven effective for a variety of applications including:
Detection of SARS-CoV-2
Case Study - Collaborative R&D, Leicester University
With funding from an MRC CiC award and supported by the Leicester Drug Discovery and Diagnostics team, scientists at the University of Leicester collaborated with members of NEB’s Research Team during development of a SARS-CoV-2 assay based on reverse transcription loop-mediated isothermal amplification (RT-LAMP). This included in-depth discussion of the enzyme formulation and primers to be used in the assay. Initial lab tests were performed using the WarmStart® LAMP Kit and WarmStart® Colorimetric LAMP 2X Master Mix. This successful collaboration led to implementation of a customised fluorescent detection assay sufficient for 10,000 asymptomatic screening test, offered to staff and students at the University of Leicester between October and December 2020. The final implemented assay was performed to a quality managed workflow with support from Leicester Molecular Diagnostics and University Hospitals of Leicester Pathology Services with positive samples feeding into the NHS England Test and Trace system (Pillar 1).
LAMP for other infectious diseases
Testing raw sewage
Other applications
LAMP is suitable for use in a wide variety of amplification-based molecular diagnostic assays. Contact us to discuss your technical requirements. You can find all the Application Notes for LAMP here.
Scale-up
- NEB has responded to the COVID-19 pandemic by scaling production to meet increasing global customer needs.
- We can offer rapid and flexible in-house scale-up capabilities.
- WarmStart® LAMP Kit (DNA & RNA) #E1700: This reagent has been incorporated into Oxford Nanopore’s LamPORE technology, a combination of LAMP with nanopore sequencing, initially implemented to provide a rapid, low-cost and scalable assay for the detection of SARS-CoV-2.
Ease of setup
- LAMP offers ease of setup for a test that can be administered in varied settings, without the need for a laboratory.
- Kits with primers included eases development of your commercialized product. We work with multiple UK and European service diagnostic providers to support their technology and supply them with LAMP reagents at scale.
- LAMP primers can be challenging to design manually, and software programs are strongly recommended. We have developed the NEB LAMP Primer Design Tool to ease both design of LAMP primers and likelihood of reaction success.
UK-based project management for an end-to-end tailored solution
We can provide you with our full range of products in custom formulations, aliquots and formats, with full support from our specialised teams and a personal manager for your project, based in the UK. We fully support you through development, proof of concept, regulatory process, scale up and launch, with tailored capabilities at each stage. NEB is vastly experienced in offering our products on an OEM basis. Download the Brochure or visit our Customized Solutions page to find out more.
LAMP Products
All our LAMP products are available in bulk formats.
Products for Fluorometric LAMP
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Products
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Product Notes
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Custom Formulations Available
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WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG)
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• Simple, one-step solution for LAMP and RT-LAMP
• Reduce risk of carryover contamination with thermolabile UDG and dUTP included in the master mix
• Includes fluorescent dye to enable real-time fluorescence measurement of LAMP/RT-LAMP reaction
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• Contact us to discuss lyophilization options
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WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)
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• Simple, one-step solution for LAMP and RT-LAMP
• Reduce risk of carryover contamination with thermolabile UDG and dUTP included in the mix
• Fully buffered master mix is compatible with different sample types and supports multiple detection methods
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• Contact us to discuss lyophilization options |
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WarmStart LAMP Kit (DNA & RNA)
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• Master mix for LAMP and RT-LAMP workflows
• Supports multiple detection methods, including fluorescence and turbidity
• Automation compatible
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• Lyophilization-ready (custom formulation available)
• High concentration
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Bst 2.0 WarmStart DNA Polymerase
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• Improved reaction properties compared to wild-type Bst DNA Polymerase
• Increased dUTP tolerance enables carryover prevention
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• Glycerol-free
• High concentration
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WarmStart RTx Reverse Transcriptase
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In-silico designed RT for RT-LAMP with reversibly-bound aptamer that inhibits activity below 40°C
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• Glycerol-free
• High concentration
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Products for Colorimetric LAMP
Related Products
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Products
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Product Notes
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Custom Formulations Available
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Thermolabile Proteinase K
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• Unique thermolabile version is completely inactivated in typical isothermal and RT-qPCR workflows
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• High concentration
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LAMP Fluorescent Dye
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• Enables real-time fluorescence measurement of a LAMP reaction
• Component of the WarmStart® LAMP Kit (DNA & RNA) (NEB #E1700)
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Monarch® Total RNA Miniprep Kit
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• Successfully used on saliva and buccal swabs as well as simulated nasopharyngeal samples. Also works with tough to lyse samples (bacteria, yeast, plant)
• New streamlined protocol enables extraction from saliva in under an hour
• Effectively purifies total RNA of all sizes, including small RNAs >20 nt
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• Please enquire if you have any special requirement
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| Monarch® RNA Cleanup Kit |
• Successfully used for viral RNA extraction from saliva, buccal swabs, and simulated nasopharyngeal swabs, with the addition of DNA/RNA Protection Reagent (NEB #T2011).
• Extremely fast – extraction complete in under 10 minutes.
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Deoxynucleotide (dNTP) Solution Mix
Deoxynucleotide (dNTP) Solution Set
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• Highly pure
• Ultrapure: ≥ 99% as determined by HPLC analysis
• Exceptional stability
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• Custom concentration
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NEB products are for Research Use only.
This content is covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at busdev@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
Your next step
Whatever your level of interest, or the scale of your project, please contact us:
Phone: 0800 318486
email: custom.uk@neb.com